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olig2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc olig2
    Olig2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+olig2/Olig2+XP+Rabbit+mAb/pmc12996796-121-31-32
    Average 95 stars, based on 57 article reviews
    olig2 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Therapeutic targeting of differentiation-state dependent metabolic vulnerabilities in diffuse midline glioma
    Article Snippet: Antigen retrieval was performed in sodium citrate buffer (2.94 g sodium citrate, 500 μl Tween 20, pH 6.0) and slides were maintained at a rolling boil for 20 min. After cooling, tissue sections were blocked in 2.5% bovine serum albumin (Sigma; #A2153), 0.2% Triton X‐100, in PBS for 1 h at room temperature. .. Sections were incubated overnight at 4 °C with primary antibodies diluted in blocking buffer: anti-OLIG2 (CST; #65915; 1:100), anti-KI67 (Abcam; #ab15580; 1:1000), anti-CC3 (CST; #9664; 1:100), anti-GFAP (Dako; #Z0334; 1:1000), anti-S100 (Dako; #GA50461-2; 1:200). ..

    Article Title: Blood-Derived α-Synuclein Aggregated in the Substantia Nigra of Parabiotic Mice
    Article Snippet: .. Primary antibodies anti-α-synuclein (Abcam, ab155038, Cambridge, UK), anti-olig2 (CST, 17198, Boston, MA, USA), anti-Iba1 (CST, ab109186, Boston, MA, USA), and anti-β-actin (Bioss, bs-0061R, Beijing, China) were incubated overnight at 4 °C and washed. ..

    Article Title: Inducible Pluripotent Stem Cell-Derived Small Extracellular Vesicles Rejuvenate Senescent Blood-Brain Barrier to Protect against Ischemic Stroke in Aged Mice.
    Article Snippet: .. Brain slices or cells were washed with PBS, permeabilized with Triton X-100 solutions (Solarbio, T8200, CN), blocked with 5% bovine serum albumin (BSA, Sigma, USA), and then incubated with primary antibodies overnight at 4°C including anti-CD3 (Abcam, ab16669, UK), anti-F4/80 (Abcam, ab6640, UK), anti-MPO (Abcam, ab9535, UK), anti-NeuN (Cell Signaling Technology, 24307, USA), anti-Olig2 (Cell Signaling Technology, 65915, USA), anti-GFAP (GB12096, Servicebio, CN), anti-Iba1 (Abcam, Ab178847, UK), anti-MAP-2 (Cell Signaling Technology, 4542s), Nanog (Abcam, ab109250, UK), Oct4 (Abcam, ab19857, UK), SSEA4 (Abcam, ab16287, UK), Tra-1-60 (Abcam, ab16288, UK), Tra-1-81 (Abcam, ab16289, UK), CD31 (R&D Systems, AF3628, USA), anti-Claudin5 (Invitrogen, 34-1600, USA), anti-ZO1 (Invitrogen, 61-7300, USA), p16INK4a (Abcam, ab189034, UK), γ-H2AX (Cell Signaling Technology, 9718s, USA). .. Next, associated secondary antibodies were incubated for 1 hour at room temperature, followed by DAPI (C1002, Beyotime Biotechnology, Beijing, CN) staining and slice mounting.

    Blocking Assay:

    Article Title: Therapeutic targeting of differentiation-state dependent metabolic vulnerabilities in diffuse midline glioma
    Article Snippet: Antigen retrieval was performed in sodium citrate buffer (2.94 g sodium citrate, 500 μl Tween 20, pH 6.0) and slides were maintained at a rolling boil for 20 min. After cooling, tissue sections were blocked in 2.5% bovine serum albumin (Sigma; #A2153), 0.2% Triton X‐100, in PBS for 1 h at room temperature. .. Sections were incubated overnight at 4 °C with primary antibodies diluted in blocking buffer: anti-OLIG2 (CST; #65915; 1:100), anti-KI67 (Abcam; #ab15580; 1:1000), anti-CC3 (CST; #9664; 1:100), anti-GFAP (Dako; #Z0334; 1:1000), anti-S100 (Dako; #GA50461-2; 1:200). ..

    other:

    Article Title: RACK7 Interacts with PRC2 Complex to Regulate Astrocyte Development
    Article Snippet: The primary antibodies used were listed as below: Anti‐PRKCBP1/RACK7(A302‐089A, Bethyl), Anti‐histone H3K27ac (ab4729, Abcam), Anti‐histone H3K4me1 (39297, Active motif), Anti‐histone H3K4me3 (#9727, Cell signaling technology), Anti‐histone H3K36me3 (#4909, Cell signaling technology), Anti‐histone H3K27me3 (#9733, Cell signaling technology), Anti‐Histone H3 (ab18521, Abcam), Anti‐Histone H4 (#13919, Cell signaling technology), Anti‐Ezh2(#5246, Cell signaling technology), Anti‐EED (#85322, Cell signaling technology), Anti‐SUZ12 (#3737, Cell signaling technology), Anti‐RBBP4 (20364‐1‐AP, Proteintech), Anti‐Lamin B1 (66095‐1‐Ig, Proteintech), Anti‐GFAP (16825‐1‐AP, Proteintech), Anti‐GFAP (#80788, Cell signaling technology), Anti‐NeuN (#24307, Cell signaling technology), Anti‐Nestin (ab6142, Abcam), Anti‐OLIG2 (#65915, Cell signaling technology), Anti‐S100 beta (ab52642, Abcam).

    Staining:

    Article Title: Spatial epigenomic niches underlie glioblastoma cell state plasticity
    Article Snippet: Cells were then permeabilized and blocked using 5% Normal Goat Serum (Cell Signaling, 5425) in DPBS containing 0.3% TritonTM X-100 for one hour. .. Primary antibody staining was performed overnight at 4 °C using anti-OLIG2 (Cell Signaling, 65915) diluted 1:400 in DPBS supplemented with 1% Bovine Serum Albumin (BSA) and 0.3% TritonTM X-100 (pH 8.0). .. Following primary antibody incubation, cells were incubated with Alexa Fluor 647-conjugated goat anti-rabbit secondary antibody (Invitrogen, A21244), diluted 1:500 in the same buffer, for one hour at room temperature.



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